Liposome fusogenic enzyme-free circuit enables high-fidelity determination of single exosomal RNA

Liposome fusogenic enzyme-free circuit enables high-fidelity determination of single exosomal RNA

Blog Article

Accurate determination of single exosomal inclusions in situ presents a significant challenge due to their extremely low abundance as well sub-100 nm vesicle dimensions.Here, we created a Liposome Fusogenic Enzyme-free circuit (LIFE) approach for the high-fidelity identification of exosome-encapsulated cargoes without destroying the vesicle integrity.The probe-loaded cationic fusogenic liposome could product capture and fuse with a single target exosome, enabling probes delivery and target biomolecule-initiated cascaded signal amplification in situ.Then the DNAzyme probe encountered conformal change upon exosomal microRNA activation, and generated a convex DNAzyme structure to cleave the RNA site of substrate probe.

After that, the target microRNA could be released to introduce a cleavage cycle to yield amplified fluorescence click here readout.Therefore, trace cargoes in a single exosome could be accurately determined by elaborately controlling the ratio of introduced LIFE probe, paving the way toward the exploration of a universal sensing platform for the assessment of exosomal cargoes to facilitate early disease diagnosis and personalized treatment.